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Inglés > Español: Un articulo cientifico (química) de 4535 palabras

un articulo cientifico
4535
14/07/17

Ejemplo de texto:
Xylanases are hydrolytic enzymes responsible for the depolymerization of xylan, and they are of great
interest because of their wide industrial applications. Extracellular low molecular weight xylanase was
purified from a submerged culture of a thermophilic fungus, Scytalidium thermophilum ATCC No. 16454
grown on corn cobs, and the enzyme was characterized. Among the investigated low-cost purification
techniques, the highest recovery (79%) with 2.7-fold purification, was obtained using an aqueous two
phase system. The maximal purity (4.3-fold) was achieved by ultrafiltration and generated a yield of
25%. A two-step chromatography procedure consisting of gel filtration and anion exchange without
any additional pre-purification steps yielded ca. 21.8-fold xylanase purification to apparent homogeneity
with 9.6% recovery as demonstrated by SDS-PAGE. This study is the first to report a complement
purification procedure and the corresponding results for S. thermophilum low molecular weight xylanase
isolation from culture medium. The enzyme has a molecular weight of 21 kDa and an isoelectric point
of pH 8.6, indicating that it belongs to GH family 11. The low molecular weight of the enzyme provides
the important advantage of making it easy to access in the lignocellulosic network, and it efficiently
degrades hemicellulose.

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